Purpose: Recreational and competitive running are popular sport activities in the United States. In 2016, approximately 65.1 million Americans participated in running activities, 9.17 million between the ages of 18 and 24 years. Although recently published studies have examined the effect of films, focusing on themes such as violence and gun use, smoking and alcohol use, and sexual activity, on risk-taking behaviors and choices made by children and adolescents after media exposure, none have examined the content of films associated with running and track & field. The objective of this study is to determine the positive and negative themes found in a select number of running-related films. Methods: We conducted a prospective cohort study examining positive and negative themes found in a select number of running-related films as identified by several popular websites. Each film was independently viewed and scored by four reviewers, and events associated with a positive or negative theme were noted. Results: A sample of 31 running-related films were analyzed. The average number of positive and negative themes was 31.5 and 9.1 mean events per hour for all included films, respectively. The most common positive themes were “overcoming physical limitations/obstacles/injuries/pain”, “overcoming mental obstacles/fear/stress”, and “praise/encouragement from family/friends/fans” (2.99, 2.73, 2.39 mean events per hour, respectively). The most common negative themes were “giving up/failing to try/losing confidence”, “ignoring injuries/running while injured”, “discouragement/ridicule/taunting from family/friends/fans” (1.29, 1.22, 0.75 mean events per hour, respectively). Conclusions: Based on our sample of running-related films, the number of positive themes far outweigh negative themes. We encourage the co-viewing and active mediation of running-related films among pediatric athletes and their coaches, athletic trainers, and/or parents in order to focus on “teachable moments” that may encourage the acquisition and development of positive themes and the avoidance and de-emphasis of negative themes.